In Marsha's clinical case, direct microscopic observation of acid-fast bacteria in sputum, combined with slow culture growth forming colorless granulated colonies and molecular verification using nucleic acid amplification and DNA sequencing, finalized her diagnosis of *Mycobacterium tuberculosis* infection. She recovered completely after $${}9$$ months of treatment.

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In Marsha's clinical case, the doctor suspected tuberculosis due to her symptoms and potential exposure to *Mycobacterium tuberculosis* in a Nigerian medical lab. To prevent transmission, she was ordered to isolate at home in a single room, wear a respiratory mask, and take a semester off school. She was prescribed a drug cocktail of isoniazid and rifampin to be taken three times daily for at least three months.

Clinical Focus: Tuberculosis Treatment and Quarantine

https://openstax.org/details/books/microbiology/

Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology.

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